By Kevin D. Altria (auth.)
During the 1980's the research of prescription drugs was once ruled by way of excessive Perfor mance Liquid Chromatography (HPLC). different separative recommendations akin to gasoline Chromato graphy and skinny Layer Chromatography provided possible choices yet their quantitative capabili ties and/or solute variety couldn't procedure that of HPLC. nearly all of prescribed drugs are ionic and it might be moderate to imagine that electrophoresis could be important within the research of prescription drugs. notwithstanding, the electrophore tic tools on hand within the 1980's have been labour extensive and hired post-separation detection strategies. in the course of the past due 1980's and early 1990's wide examine used to be con ducted into the probabilities of engaging in electrophoretic separations in capillaries. This technique allowed online detection and will be played on absolutely automatic gear. This study ended in the arrival of contemporary day capillary electrophoresis (CE) tools which provide related functionality and automation degrees to that of HPLC. learn used to be additionally interested by constructing functions for CE and specific cognizance used to be paid to purposes in the pharmaceutical research zone. those functions proved that CE will be utilized to quite a lot of drug kinds together with water insoluble and impartial compounds. the power to accomplish effective chiral separations of substances additionally elevated the recognition of the approach. CE with oblique UV detection has turn into tested as an easy and powerful replacement to ion-exchange chromatography for the selection of small inorganic or natural ions.
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Extra resources for Analysis of Pharmaceuticals by Capillary Electrophoresis
Altria KD, Ed. Capillary Electrophoresis Guidebook - Principles, Operation and applications, Humana Press, Totowa, 1995. 6. H Watzig, Appropriate calibration functions for capillary electrophoresis I. Precision and sensitivity using peak areas and heights, J. Chromatogr. A, 700 (1995) 1-7. 7. Terabe S, Otsuka K and Ando T, Electrokinetic chromatography with micellar solution and opentubular capillary, Anal. , 57 (1985) 834-841. 8. Altria KD, Bryant SM and Hadgett T, Validated capillary electrophoresis method for the assay of a range of acidic drugs and excipients, J.
The factors affecting CE precision (and to a lesser extent accuracy) are widely different to HPLC which is currently the predominantly employed analytical technique for drug assay. The main difference is that HPLC employs a well controlled fixed volume injector system whilst a time and pressure dependent hydrodynamic injection is generally employed in CEo In addition, sample solution viscosity is an important factor in CE as it greatly effects the volume of sample injected into the capillary whilst viscosity has no influence on HPLC injection volumes.
5, 25 mM phosphate buffer has been validated (1) for assay of a wide range of basic drugs. 3-2 % for peak area ratios. The robustness of the method to deviations in the method settings was satisfactorily assessed using an experimental design (see Chapter 14 for details on the use of experimental designs). Shelf-lives of electrolyte and sample solutions were assessed (3 months and 14 days, respectively). The analysis was repeated on different days, by different analysts on different equipment using different reagents and samples.
Analysis of Pharmaceuticals by Capillary Electrophoresis by Kevin D. Altria (auth.)