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Read e-book online Analytical ultracentrifugation PDF

By Cole, James L

ISBN-10: 0128029080

ISBN-13: 9780128029084

ISBN-10: 0128029099

ISBN-13: 9780128029091

Analytical Ultracentrifugation, the newest quantity in Methods in Enzymology, makes a speciality of analytical ultracentrifugation. The scope of this method has tremendously multiplied lately as a result of advances in instrumentation, algorithms and software program.

This quantity describes the newest techniques within the box and within the functions of analytical ultracentrifugation within the research of macromolecules, macromolecular assemblies, and biopharmaceuticals.

  • Timely contribution that describes a speedily altering field
  • Leading researchers within the field
  • Broad insurance: instrumentation, easy thought, facts research, and applications

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Statistical analysis: To gain a better appreciation of the different noise levels present in these disparate datasets, we performed a statistical analysis of the fitting results as a function of total concentration. It is reasonable to expect different noise levels at different concentrations on different instruments; hence, we compared the root mean square deviations (RMSDs) obtained from the degenerate 2DSA analysis of the iterative refinement step, which produced random residuals in all cases.

Gorbet et al. ACKNOWLEDGMENTS We acknowledge the invaluable contributions from our colleagues at the Texas Advanced Computing Center, in particular Chris Hempel, who have provided important assistance with the use of XSEDE supercomputing resources. We also would like to thank the Science Gateway group at Indiana University (Marlon Pierce, Raminder Singh, and Suresh Marru) for their help with implementing the parallel submissions. D. P. C. acknowledge financial support by the Center for Applied Photonics (CAP) at the University of Konstanz.

Demeler, B. (2007). Parsimonious regularization using genetic algorithms applied to the analysis of analytical ultracentrifugation experiments. In GECCO proceedings ACM 978-1-59593-697-4/07/0007. , & Demeler, B. (2008). Parallel computational techniques for the analysis of sedimentation velocity experiments in UltraScan. Colloid & Polymer Science, 286(2), 138–148. , & Demeler, B. (2005). Modeling analytical ultracentrifugation experiments with an adaptive space-time finite element solution of the Lamm equation.

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Analytical ultracentrifugation by Cole, James L

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