By John H. Hash
The seriously acclaimed laboratory regular, equipment in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. because 1955, each one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really a necessary e-book for researchers in all fields of lifestyles sciences.
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B. Transduction . . . . . . . . C. Transformation . . . . . . . III. Enhanced Segregation of Resistance Plasmids by Curing Agents A. Acridine Orange, Quinacrine, and Ethidium Bromide Curing B. Sodium Dodecyl Sulfate . . . . . . C. Mitomycin . . . . . . . . IV. Compatibility Properties of R Plasmids . . . . A. Determination of fi Character . . . . . B. Testing for Compatibility of R Factors . . . . . . . . . . . . . . 41 43 43 45 48 49 51 51 52 52 54 54 I.
Coli and Streptomyces. More recently a procedure that significantly improves the percentage of mutants among survivors was described by Townsend et al. TM for Streptomyces. In this case, near UV light in the presence of the photosensitizing chemical, 8-methoxypsoralen was employed. A longwave UV lamp (emitting at >3000 A), commonly referred to as "black light," is set up as previously described for UV treatments. The 8-methoxypsoralen is dissolved in ethanol at 1 mg/ml, and then diluted 1:10 in a water suspension of spores; exposure times of 10-60 min appear to be adequate.
What may be considered a classic in this field is the work of Backus and Stauffer1 with Penicillium chrysogenum at the University of Wisconsin. This paper is important not only because it reports an increase in penicillin yields 40 times greater than those produced by the parent culture, but primarily because it presents an overall view of the techniques and approach followed over a 10-year period. II. Fermentation Conditions These will not be discussed in any detail here; suffice it to say that with a mutation program a parallel study should be initiated to develop satisfactory fermentation conditions.
Antibiotics by John H. Hash