By P. Michael Conn, Anthony R. Means (Eds.)
The seriously acclaimed laboratory usual, Methods in Enzymology, is among the such a lot hugely revered courses within the box of biochemistry. when you consider that 1955, every one quantity has been eagerly awaited, often consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable this day - actually a necessary book for researchers in all fields of existence sciences
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Extra resources for Cellular Regulators Part B: Calcium and Lipids
Philips) but their large diameter (12 mm for the Philips electrode) precludes their use in sample volumes smaller than 5 ml, a severe drawback in studies of cellular and subcellular biochemistry where the amount of biological material is often limited. In the following we describe the preparation and use of liquid membrane minielectrodes selective for Ca ~+. They have a small diameter (1 to 3 mm) and can be easily assembled with inexpensive, commercially available materials. 5 sec) and the high sensitivity of the minielectrode, it is essential to use an optimized incubation vessel.
Tan and F. Q. Nuttall, Biochim. Biophys. Acta 410, 45 (1975). ~1 G. T. Cori and A. A. Green, J. Biol. Chem. 151, 31 (1943). 20 MEASUREMENT AND PERTURBATION OF CALCIUM  either glycogen breakdown 9 or incorporation of glucose 1-phosphate into g l y c o g e n . 12A3 The level of phosphorylase a depends on the relative activities of phosphorylase kinase and various phosphatases. 14-18 The activity of the kinase can be regulated by calcium and cAMP. Calcium activates the phosphorylase kinase, in a noncovalent manner, by interacting with the calmodulin-like 8 subunit of the enzyme.
Ballou, J. Biol. Chem. 236, 54 (1961). 6t R. V. Tomlison and C. E. Ballou, J. Biol. Chem. 236, 1902 (1961). 62 R. F. Irvine, K. D. Brown, and M. J. Berridge, Biochem. J. 221, 269 (1984). 4 W Insll,4~5)P3 FIG. 3. Ins(1,4,5)P3-induced Ca 2+ release from the SR of skinned fibers. Redrawn with permission from Volpe e t al. 56 A segment of a chemically skinned fiber of the rabbit adductor is loaded with Ca 2+ (pCa 6A), rinsed in solution W, and then challenged with Ins(1,4,5)P3: 1 and 20/xM in the upper and lower trace, respectively.
Cellular Regulators Part B: Calcium and Lipids by P. Michael Conn, Anthony R. Means (Eds.)